Close to a year after their reunion, Anna and Elsa are finally getting back into truly living life. However as things look up Elsa disappears and strange things start to happen. It might take Anna and Kristoph's shaky trust in two strangers to save the Kingdom once again.

Professor Layton receives a mysterious package from his ex-assistant Emmy citing that something is happening and she needs his help to stop it. Inside is an old relic that Emmy tells Layton to guard with his life and to meet her at her home. But even walls have ears and Layton and crew will find themselves alongside allies both new and old in one of their greatest quests for truth.