Toad is very fond of his mother; Princess Peach, but he also has a relationship with his grandfather. When Peach leaves to attend a meeting, Toadsworth is left to look over his grandson.

Toad and his siblings are visited by a familiar foe, who will stop at nothing to get his revenge on the Royal Mushroom Baby and his family.

Toad and his family travel to New Donk City to visit a friend, but come into contact with four mutated turtles.